Utilisation de la spectrométrie de masse VITEK MS PRIME pour l'identification en routine des agents responsables de la sporotrichose à partir de cultures de biopsie cutanée
Speaker

Tahinamandranto Rasamoelina
Madagascar
Depuis 2019, Tahinamandranto Rasamoelina est Responsable Scientifique du Centre d’Infectiologie Charles Mérieux de la Faculté de Médecine de l’Université d’Antananarivo, Madagascar. Il est diplômé de Doctorat en Biotechnologie, Instrumentation, Signal et Imagerie pour la Biologie, la Médecine et l’Environnement préparé au sein de la communauté Université Grenoble Alpes. Il a depuis 10 ans travaillé sur les mycoses profondes (Chromoblastomycose, Sporotrichose et mycétome) avec les dermatologues du CHU de Befelatanana et l’équipe mycologue et parasitologue du laboratoire TIMC de l’Université Grenoble Alpes. Depuis 2020, il est la personne focale sur le diagnostic de la lèpre et la détection des résistances aux antilépreux. Il est parmi les co investigateurs principaux sur sites aux études collaboratives sur la bilharziose à Madagascar réalisées avec les partenaires nationaux et internationaux
Source event

WSHD Madagascar | XIXth International Congress of Dermatology
For its XIXth Congress, and IIIrd WSHD, the SOMADER choose his topic of Neglected Tropical Diseases (NTDs) which are groups of parasitic and bacterial infections affecting over a billion people worldwide, mainly in low-income areas. These diseases often cause chronic and debilitating health conditions, which severely affect the quality of life of those affected, leading to social exclusion and poverty.
Of the approximately 20 NTDs established by the World Health Organization (WHO), twothirds of them have skin manifestations. The Skin NTDs can cause severe morbidity, disability, and disfigurement if left untreated. These conditions include Buruli ulcer, Cutaneous leishmaniasis, Post kala-azar dermal leishmaniasis, Leprosy, Lymphatic filariasis, Mycetoma, Onchocerciasis, Scabies and other parasitic infections of the skin, Yaws, Chromoblastomycosis, and Sporotrichosis. we are working to include chronic inflammatory diseases such as atopic dermatitis (AD) in the category of cutaneous NTDs, where the incidence is rising dramatically in sub-Saharan Africa.
To address the neglected burden of skin NTDs, the SOMADER organize this Congress in Toamasina on May 22 and 23, 2024 with the participation of speakers from several countries, in collaboration with Global Dermatology, WHO, ISAD and ILDS. The event will
showcase over 40 presentations in English and French, with subtitles available in several languages, including French, Spanish and English. On May 24-25, a field visit to primary health care centers is planned around Tamatave, an area hyperendemic in chroblastomycosis and sporotrichosis. Teams from Grenoble’s mycology-parasitology laboratory have already confirmed their participation. You can visite our WSHD last event’s on global dermatology website.
This event is an excellent initiative that can help raise awareness of skin NTDs and the importance of early diagnosis and treatment. By bringing together experts from various countries, the event can provide a platform for sharing experiences, best practices, and innovative approaches to combat these diseases. It is a step towards strengthening global partnerships and collaborations to eliminate skin NTDs and improve the health and wellbeing of millions of people worldwide.
The burden of skin NTDs is significant, and concerted efforts are needed to address this public health challenge. We also give our guests the opportunity to visit the city of Toamasina, the economic heart of Madagascar.
Description
Introduction : La sporotrichose est une infection fongique due à un champignon dimorphique Sporothrix schenckii. L’une des caractéristiques de la maladie est la propagation des lésions surélevées et ulcérées tout au long des vaisseaux lymphatiques. Le diagnostic biologique de la maladie repose sur les examens mycologiques à partir des biopsies afin de permettre l’orientation de la détection mais pour confirmer l’espèce, il est indispensable de faire recours aux techniques moléculaires dont PCR, séquençage et la spectroscopie de masse MALDI-TOF MS. Cette dernière technique est une méthode rapide, facile, rentable et fiable dans l’identification d’un large éventail de micro-organisme. Dans cette étude, les appareils de spectrophotométrie utilisés étaient MALDI Biotyper® et VITEK® MS PRIME Objectif : Comparer les résultats obtenus entre MALDI Biotyper® et VITEK® MS PRIME Matériels et méthodes : Dans cette étude, 13 isolats de colonies de culture de Sporothrix schenckii tous positif à la PCR spécifique ont été testés pour les systèmes MALDI Biotyper® et VITEK® MS PRIME. Cinq isolats supplémentaires ont été testés uniquement sur le système VITEK® MS prime. Parmi ces isolats, 11 échantillons de la région ITS ont été séquencés. Toutes les colonies ont été cultivées sur milieu Sabouraud Chloramphénicol solide puis incubées à 30°C pendant 2-7 jours. Ensuite, suite aux dépôts des souches sur les spots de la cible de chaque appareil, l’acide formique a été ajouté avant la matrice. Le lancement des analyses s’ensuit la manipulation et les résultats ont été lus suivant des scores et des codes couleurs d’identifications. Une comparaison des résultats obtenu entre les techniques a été effectuée. Résultats : Une similarité de 100% (13/13) a été noté entre les résultats PCR et MALDI Biotyper® tandis qu’une similarité 89% (16/18) entre PCR et VITEK® MS PRIME. Seuls les résultats de deux échantillons diffèrent entre MALDI Biotyper® et VITEK® MS PRIME donnant un taux de similarité 85% (11/13). Les deux systèmes ont obtenu un accord de 100% au niveau du genre. L’identification au niveau de l’espèce par MALDI Biotyper® et VITEK® MS PRIME était 92,3 % (12/13) et 100% (18/18) respectivement. Parmi ce dernier, 16,6% (3/18) était à faible discrimination. Conclusion : Les deux systèmes ont exposé d’excellents taux d’identification de l’agent Sporothrix schenckii. Ils sont performant pour l’identification de l’espèce. Cependant, une mise au point supplémentaire sur la manipulation et une augmentation des nombres d’échantillon à traiter serait envisageable pour améliorer la qualité de l’identification sur l’appareil VITEK® MS PRIME. Mots clés : Sporotrichose, Sporothrix schenckii, spectrométrie de masse
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DISCUSSIONS
CHROMOBLASTOMYCOSIS, SPOROTRICHOSIS AND MYCETOMA
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Utilisation de la spectrométrie de masse VITEK MS PRIME pour l'identification en routine des agents responsables de la sporotrichose à partir de cultures de biopsie cutanée

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